This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017. MWR is excited to offer a two-year comprehensive fellowship in vitreoretinal diseases and surgery beginning July, 2021 and ending July, 2023. Future study will evaluate the quality of protein detection with ELISA assays after using these methods Founded in 2002, Midwest Retina (MWR) has grown quickly into the largest retina-only private practice in Central Ohio. Vitreous sample sonication or lysis buffer extraction significantly improves the consistency of protein measurement of samples. These were not statistically significant compared to control (p=0.3988 and p=0.2464, respectively). Midwest Retina Consultants, Arlington Heights, IL. School of Optometry and Vision Science Midwestern University, Arizona College. The mean CV of vortex treatment and hand-held homogenization were 17.05% ± 7.73 and 18.43% ± 3.28, respectively. There are also frequent Chicago retina meetings and/or dinners that the fellow will be encouraged to. American Society of Retina Specialists American Academy of Ophthalmology. The retinal lipids of rats fed MCR oils showed higher levels of total cholesterol, triacylglycerols and phospholipids when compared to rats fed oils containing. The mean CV of lysis buffer treatment and sonication are significantly lower than that of control (4.34% ± 1.79, P=0.0417 and 4.49% ± 3.14, p=0.0449, respectively). The mean CV for the no treatment control was 13.48% ± 9.21. Student’s t-test was used for statistical analysis. The coefficient of variation (CV %) from the three assays was averaged between the 4 subjects and compared between the 5 methods. Samples in each assay were run in triplicate accounting for pipetting error. Three independent assays were run with each treatment and all 4 subjects. BCA assay with 96 well plates was performed for the comparison of five groups of vitreous preparation: 1) vortex (S3200, BioExpress), 2) hand held-homogenization (Disposable pellet mixer and cordless motor, VWR), 3) sonication (Dismembrator-500, Fisher Scientific), 4) the use of lysis buffer (9803S, Cell signaling), and 5) no treatment as a control. Simhaee looks forward to meeting you and developing a connection that will. Charter Inductee of the Retina Hall of Fame 2017 OSN Top 150 of Retina Specialist 2016 American Academy of Ophthalmology Senior Achievement Award, 2011. Patient vitreous samples (n=4 /group) were randomly selected from sample collection under an IRB-approved protocol during retinal detachment surgical procedure. NYU Grossman School of Medicine Residency, Ophthalmology, 2014 2017. Bhatnagar currently presides over the Retinal Service at Albany Medical Centers Ophthalmology Service in addition to serving as a preceptor for the retina. The purpose of this study is to compare multiple methods of vitreous sample preparation for the consistency of measured protein levels between multiple assays. Vitreous structure can hinder the measurement of consistent protein concentration levels over subsequent assays for research or clinical testing in retinal detachment.
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